The difference between different extraction solvent applications of chlorophyll meter

Chlorophyll is the main photosynthetic pigment of plant leaves. Chlorophyll content is an important indicator in the study of plant physiology. Chlorophyll content determination methods include spectrophotometer method, living chlorophyll meter method and photoacoustic spectroscopy. However, using the handheld chlorophyll meter to measure the relative content of pigments, and the relationship equation between the pigment index and the actual chlorophyll content in different types of plant leaves is different, that is, a relationship cannot be used to convert the pigment content of different plants.

A chlorophyll meter was used to measure the chlorophyll content of the Chinese cabbage, and the fresh Chinese cabbage leaf was washed, and the surface moisture was sucked with absorbent paper. After the large veins were removed, the leaves were cut into small pieces of about 0.2cm×1.0cm. Filament, mix and spare. Weigh 0.1g of the above sample into a mortar, add a small amount of quartz sand and 80% acetone, dilute to 20ml, filter, and repeat the chlorophyll meter determination three times.

The chlorophyll content in the extract was determined after 1, 2, 5, and 8 days at room temperature. From the chlorophyll meter value table, it can be seen that except for the B treatment, the chlorophyll a, chlorophyll b, and the total amount of the other treatments all showed a decreasing trend with time; the chlorophyll a, chlorophyll b, and the total amount of the treatment B reached the maximum on the second day. The value, which may be due to the slower acetone extraction rate.

Chlorophyll meter showed that chlorophyll a decreased by 1.76% to 8.31%, chlorophyll b decreased by 1.50% to 5.60%, and total chlorophyll decreased by 1.69% to 7.44 after the chlorophyll extractor was placed 8 days. %. This shows that chlorophyll b is more stable than chlorophyll a in these extracts. The total amount of chlorophyll decreased only 1.69% at the 8th day after treatment E, which was the lowest.

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