High-performance liquid chromatography is one of the common instruments in the experiment. The sensitivity of the column is an important reason to ensure the accuracy of the experiment. How can we solve the problem when the sensitivity of the column is not enough? It should be analyzed from the following aspects:
1. Insufficient sample size: the solution is to increase the sample size.
　　 2 , the sample does not flow out of the column: the mobile phase or column can be changed according to the chemical nature of the sample
　　 3 , the sample does not match the detector: adjust the wavelength according to the chemistry of the sample or change the detector
　　 4 , the detector attenuation is too much: adjust the attenuation.
　　 5 , the detector time constant is too large: the solution is to reduce the time parameter
　　 6. Detector pool window pollution: The solution is to clean the pool window.
　　 7. There are bubbles in the detection tank: the solution is exhaust.
　　 8. The measuring range of the recorder is improper: adjust the voltage range.
　　 9. The flow rate of the mobile phase is not suitable: adjust the flow rate.
10. The detector and recorder exceed the calibration curve: the solution is to check the recorder and detector and redo the calibration curve.
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