Analysis of the Change of the Intensity of Reflected Peak in Chlorophyll Red Band with Concentration
Due to the structure and composition of chlorophyll itself, the blue light (470 nm) and red light (670 nm) of chlorophyll visible light have strong absorption. The SPAD502 chlorophyll meter was developed based on this characteristic. In the red light band, affected by the chlorophyll elastic scattering of algae, the spectral reflectance gradually increases and reaches a peak around 700 nm. As the wavelength increases, the reflectance decreases gradually due to the strong absorption of water molecules. In the red light band, the intensity of the reflection peak gradually increases with the increase of chlorophyll concentration. Figure 2 shows the change law of the intensity of the reflection peak in the red light band with increasing chlorophyll concentration.
From Fig. 2(a), it can be seen that the intensity of the chlorophyll reflex peak in the red band has a good linear correlation with the concentration, and its determination coefficient is 0.982. The chlorophyll concentration is determined by using the correlation between the chlorophyll concentration and the intensity of the reflection peak. Inversion, with good inversion effect, has an RMSE of 1.944 ug/L-1. However, when the concentration of chlorophyll reaches 10 μg·L-1, the linear correlation gradually weakens (Figure 2(b)). When the concentration of chlorophyll reaches 1 000 μg·L-1, the reflectance intensity of the red band and the chlorophyll concentration appear to be comparable. With a good logarithmic relationship [Figure 2(c)], the coefficient of determination reaches 0.965. 
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